Abstract - Nanoplastic particulates (pNP) are widely considered as being potentially harmful to the environment and living organisms while also being technically difficult to detect and identify in the presence of biological matrices. In this study, we describe a method for the extraction and subsequent Raman analysis of sub-micron plastic particulates present in the tissues of salt-water mussels. The process combines a step of enzymatic digestion/filtering to eliminate the biological matrix with a detection/identification procedure, which uses a micro-machined surface, composed of arrays holes with well-defined sub-micron depths and diameters. This sensor surface, exploits capillary forces in a drying droplet of analyte solution to drive the self-assembly of suspended nanoparticles into the holes leaving the individual particles isolated from each other over the surface. The resulting nanoparticle array, when analysed using confocal Raman microscopy, permits the size selective analysis of the individual sub-micron pNP trapped in the array structure.
